Fig. 1.

a Surface expression of CXCR3 on human CD3⁻CD56⁺ NK cells isolated from healthy peripheral blood, either directly after isolation (resting), after 24-h activation with IL-2 (activated) or after 11 days of expansion (expanded). Gray histogram represents NK cells stained with PE-conjugated anti-CXCR3 antibody. Empty histograms represent NK cells stained with IgG1-PE isotype control antibody. MFI of CXCR3 (isotype ctrl MFI) is indicated in histogram captions. One of the three representative experiments is shown. b Transwell migration assay of resting and expanded NK cells toward serum-free medium containing CXCR3 ligands, 8-µm pore size filter, 6-h incubation. Recombinant CXCL9, 10 and 11 were added to separate wells in a 24-well plate at 1 nM. One of the two representative experiments is shown