Fig. 2.

DaRT in combination with both cyclophosphamide, sildenafil with or without CpG resulted in DA3 delayed tumor growth. Mice bearing DA3 tumors (76 ± 16 mm³) were each treated with either two Ra-224-loaded (28-45 kBq/wire) or inert wires in combination with low-dose CP, sildenafil, and CpG. CP was injected i.p. 1 day before the wire insertion and at day 7 after the irradiation. Mice received sildenafil in the drinking water (20 mg/kg/24 h) for 6 weeks. CpG (100 µg) was administrated one day before wire insertion i.t and one day after wire insertion. a The tumor growth curves of DA3 tumor-bearing mice treated by either 2XRa-224 wires + CP + sildenafil, (n = 20) or 2Xinert wires + CP + sildenafil, (n = 18), P (Two-way ANOVA without replication) <0.05. *P (T test) <0.05. b The tumor growth curve of DA3 tumor-bearing mice treated by two Ra-224 + CP + sildenafil + CpG, (n = 20) or two inert wires + CP + sildenafil + CpG, (n = 20), P (Two-way ANOVA without replication) <0.05. *P (T test) <0.05