Fig. 2.

Pleural effusion CD8+ T cells from lung cancer patients stimulated with anti-CD3 mAb presented high levels of apoptosis. a Plots showing the gating strategies for lymphocytes, cells were gated on the corresponding CD4+ or CD8+ T cell subpopulation, and analysis of annexin-V versus propidium iodide (PI) was performed, data from CD3-stimulated and non-stimulated pleural effusion (PE) and peripheral blood (PB) CD4+ and CD8+ T cells from a cancer patient. b Comparison of apoptosis induced by anti-CD3 mAb stimulation (10 μg/ml) in CD4+ and CD8+ T cells from all groups. PE from non-malignant patients (n = 11), PE and peripheral blood (PB) from lung cancer patients (n = 15) and PB from healthy donors (n = 13). Bars depict the mean ± SE