FIG. 1.

Schematic diagram of RV cDNAs and the hydrophobic domain of the RV E1 glycoprotein. Respective portions of the C, E2, and E1 genes are indicated above the constructs. Intergene borders are marked by vertical lines extending through the cDNAs. The translation start site of the capsid protein is utilized in all constructs. Signal peptides are indicated by open boxes, and the transmembrane regions are shown as closed boxes. The hydrophobic domain of E1 is depicted as an open box 81 amino acid residues from the N terminus of E1. Restriction endonuclease sites are abbreviated as follows: E, EcoRI; H, HindIII; B, BamHI; and N, NcoI. The bar represents approximately 500 nucleotides. Beneath the arrows are the single-amino-acid changes and the deletion (dt) mutation. 24S, the cDNA containing all three structural protein genes of RV; E2E1, the cDNA containing the E1 and E2 genes.