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Localization of RV spike proteins by immunofluorescence. Induced BHK cells were fixed with 2% formaldehyde–PBS, incubated with monoclonal antibodies against E1 (1:50) or E2 (1:50), and then incubated with fluorescein isothiocyanate-conjugated anti-mouse immunoglobulin G. Wt, BHK-E2E1; Gly, BHK-E2E1 (G93D); Pro, BHK-E2E1(P104G); Cys, BHK-E2E1(C82S); dt, BHK-E2E1(dt). Magnifications, ×400.
