FIG. 5.

Time course of glycan processing of wild-type and mutant proteins. Induced transformed BHK cells were pulse-labelled with [³⁵S]methionine for 30 min and chased for various periods of time as indicated. Some immunoprecipitates were digested with endo-H for 8 h (+); others were not (−). E1 and E2 arrowheads indicate the E1 and E2 protein species in the absence or presence of endo-H treatment. The processing of E2 is indicated by the asterisks. The positions of molecular size markers are shown on the left (in kilodaltons). WT, BHK-E2E1; CYS, BHK-E2E1(C82S); DT, BHK-E2E1(dt).