TABLE 1.
Fusion activities of the wild-type and the mutant cell lines at different pHs
| Cell linea | Fusion activityb at pH:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 7.0 | 6.5 | 6.0 | 5.6 | 5.3 | 5.0 | 4.8 | 4.5 | 4.2 | |
| wt | − | − | + | + | ++ | +++ | ++++ | +++ | ++ |
| Cys | − | − | − | − | − | − | − | − | − |
| dt | − | − | − | − | − | − | − | − | − |
| Gly | − | − | − | − | − | − | − | − | − |
| Pro | − | − | − | + | + | ++ | ++ | + | + |
a
wt, BHK-E2E1; Cys, BHK-E2E1(C82S); dt, BHK-E2E1(dt); Gly, BHK-E2E1(G93D); Pro, BHK-E2E1(P104G).
b
The percentage of fused cells for each mutant is represented as follows: −, no fusion or less than 5% fused cells; +, 5 to 20% fused cells; ++, 20 to 40% fused cells; +++, 40 to 60% fused cells; and ++++, greater than 60% fused cells. Values are averages of data from four separate experiments.