Fig. 4.

CD133⁺ tumor-specific LN T cell treatment eradicated CD133⁺ tumor cells and induced accumulation of leukocytes in tumor tissues. One million CD133⁺ tumor cells mixed with 0.5 × 10⁶ CD133⁻ tumor cells were subcutaneously inoculated at both flanks. Zero (Gr. 1), 10 × 10⁶ (Gr. 2), 5 × 10⁶ CD133⁺ tumor-specific LN T cells (Gr. 3), or 10 × 10⁶ LN T cells draining CD133⁻ tumor antigens (Gr. 4) were intravenously infused after sublethal whole-body irradiation. Each group contained 4 mice. Four tumors were collected and digested with collagenase, DNase, and hyaluronidase 7 and 15 days after T-cell infusion. The representative data of 3 independent experiments are presented. a CD133 expression of cells in the tumor region. b Dot plots of forward and side scatter. c The percentages of CD45⁺ leukocytes based on the total number of cells (left graphs), of cells in the lymphocyte region (middle graphs), and of CD11c⁺ cells (right graphs) in each group