Fig. 1.

ELISPOT analysis of PBMC after stimulation with HDLM-2 RNA-transfected dendritic cells. Dendritic cells were transfected with total RNA from HL cell line HDLM-2 and used as antigen-presenting cells for PBMC from the same donor. On day 7, primed PBMC were re-stimulated with RNA-transfected DC. Six days after re-stimulation, the reactivity of stimulated cells against indicated cell lines was assessed by interferon gamma ELISPOT. Stimulatory cells were incubated in the absence (open bars) or presence (closed bars) of primed PBMC (50.000 cells per well). Fresh autologous PBMC (aPBMC) and medium without stimulatory cells (-) served as controls. Spots were automatically detected by a KS Elispot system (Carl Zeiss). Presented are means and standard deviations from triplicates