Fig. 5.

Oxidative stress and Foxp3(+) Tregs evaluated by immunohistochemistry. Representative immunostaining for anti-8OHdG, anti-Foxp3, anti-CD4, and anti-CD8 mAb in serial sections is shown in a. The grade of oxidative stress was classified into 8OHdG-high or 8OHdG-low groups based on the median value of the counts of 8OHdG-positive cells within tumors. Semiquantitative analysis indicated that the infiltrating grade of CD8(+) T cells was significantly down-regulated in the 8OHdG-high group compared with the 8OHdG-low group in both gastric and esophageal cancer (b). Also, the infiltrating grade of CD4(+) T cells was significantly decreased in the 8OHdG-high group compared with the 8OHdG-low group in gastric cancer (b). On the contrary, the infiltrating grade of Foxp3(+) Tregs did not differ regardless of the oxidative state in both gastric and esophageal cancer (b). Data were analyzed using a non-paired Student’s t-test