Fig. 1.

Suppression of YB-1 sensitizes renal cancer cells to IFN-α in vitro. ACHN (a), 786-O (b), and RENCA (c) cells transfected with 40 nM of the indicated siRNA were seeded into 96-well plates. The following day, various concentrations of IFN-α were added. Following incubation for 96 h, cell survival was analyzed by a cytotoxicity assay. Cell survival in the absence of IFN-α corresponds to 1. All values are representative of at least three independent experiments. Boxes, mean; bars, ±sd. d RENCA cells were transfected with 40 nM of the indicated siRNA. Cell numbers were counted at the indicated time points. The results were normalized by the cell numbers at 0 h. All values are representative of at least three independent experiments. Boxes, mean; bars, ±sd. RENCA cells were transfected with 40 nM of the indicated siRNA. At 72 h after transfection, the cells were stained with propidium iodide and analyzed by flow cytometry. The cell-cycle fractions are shown at the top right of each graph