Fig. 3.

YB-1 suppression reduces STAT3 protein level through proteasomal degradation. ACHN (a), 786-O (b), and RENCA (c) cells were transfected with 40 nM of the indicated siRNA. After 24 h, cells were treated with vehicle, 1 μg/ml of cycloheximide, or 5 μM of MG132 for additional 24 h. Whole-cell extracts were subjected to SDS–PAGE, and Western blotting was performed using the indicated antibodies