Fig. 1.

Distribution of CD3⁺CD8⁺Foxp3⁺ T lymphocytes in TIL, NIL, TDLN, and PBMC of patients with GC. a A representative flow cytometry analysis of lymphocytes in TIL and NIL of the same patients for Foxp3 versus CD25 expression after gating on CD3⁺CD4⁺ or CD3⁺CD8⁺. b The frequencies of CD3⁺CD4⁺Foxp3⁺ and CD3⁺CD8⁺Foxp3⁺ T cells in TIL and NIL from 40 GC patients (horizontal bars, mean ± SEM; each circle, single donor). c The representative dot plot of CD25⁺Foxp3⁺ cells among CD3⁺CD4⁺ and CD3⁺CD8⁺ T cells in TDLN and PBMC of the same patient. d The frequency of CD3⁺CD8⁺Foxp3⁺ T cells in matched pairs of TDLN and PBL from 19 patients. Statistical analysis in b and d was shown by Student’s paired t test. Data were the mean ± SEM