Fig. 1.

Role of isotype and FcγRIIB in anti-CD40 activity. a C57Bl/6 mice were immunised i.v. with 100 μg OVA (left) or OVA-NP (right) plus 100 μg of the indicated 3/23 or control (C) mAbs. Serum anti-OVA and anti-NP titres were determined 14 days later. b OVA-specific (OTI) CD8 T cells were transferred into wild type (WT) or FcγRIIB^−/− mice as indicated and the mice immunised as above. Levels of circulating OTI cells were determined 5 days later (primary response and right panel) or on day 60 after boosting with 100 μg SIINFEKL peptide on day 52 (memory response, centre) and are expressed as a percentage of circulating CD8+ lymphocytes. Methods were as described [42]