Fig. 2.
LZ-8 enhanced the ability of DCs to induce Ag-specific T cell activation. a Untreated (control) or LZ-8 (5 μg/ml)-treated, OVAP1 or OVAP2 (1 μg/ml)-pulsed DCs were cocultured with OT-I or OT-II T cells for 72 h. T cell proliferation was determined by [3H]thymidine incorporation (upper panels), and IFN-γ production was measured by ELISA (lower panels). b C57BL/6 mice were immunized with OVAP2 (10 μg) mixed with IFA alone (control) or IFA + LZ-8 (10 μg). Draining LN cells were collected after 10 days and cultured with indicated concentration of OVAP2 for 3 days. T cell proliferation was determined by [3H]thymidine incorporation (upper), and IFN-γ production was measured by ELISA (lower). Error bars indicate ±SD of three samples. *P < 0.05; **P < 0.01 (Student’s t-test) was comparison between LZ-8-treated and untreated cells or mice. All data are representative of two to four independent experiments