Fig. 5.

Human serum reactivity against all peptides. a Background-normalized netto reactivity (MFI) in the multiplex assay of 20 sera from patients with microsatellite-unstable colorectal cancer (filled dots) and of one serum from a patient with microsatellite-unstable colorectal cancer vaccinated with HT001(-1), TAF1B(-1), and AIM2(-1) in a clinical trial (Micoryx). The latter serum (open circles) shows strong signals against the vaccine peptides included in the assay (HT001(-1) and TAF1B(-1). b Agreement between multiplex signal and ELISA signal for all peptides measured in one serum. c Titration of the serum from the vaccinated patient. Shown is the reactivity of antibodies against HT001(-1), and TAF1B(-1)