Fig. 2.
a Up-regulation of WT1 mRNA levels in various human PC cell lines following GEM treatment. Human PC cells (1 × 106 MIAPaCa2, AsPC-1, BxPC-3, Capan-1 or Capan-2) were seeded in 10-cm culture plates. After 24-h incubation, medium was changed to media containing GEM (10, 30 or 100 ng/ml). After 48 h, we used qRT-PCR to quantify the relative ratio of WT1 to 18S mRNA levels in each cell line (n = 3). b GEM-induced up-regulation of WT1 mRNA in human PC cells with low basal levels of WT1 mRNA (MIAPaCa2, AsPC-1, BxPC-3 and Capan-1). To illustrate these results, we replotted data from (a) to represent a considerably narrower range of mRNA level ratios (0–14) on the y-axis. (c) Expression of WT1 mRNA in human PC cells with high basal levels of WT1 mRNA (PANC-1). To illustrate the results, we plotted data to represent a considerably wider range of mRNA level ratios (0–18,000) on the y-axis