Fig. 4.
Immunization and COX-2 inhibition increases systemic IFN-γ levels. N32 tumor cells were inoculated i.c. into rats and animals were immunized s.c. on day 1, 15, and 29 with irradiated N32-IFN-γ cells. Mini-osmotic pumps loaded with parecoxib (Pcb) (5 mg/kg/day) were implanted i.p. on day 7 and 17, and blood was collected from the tail vein day 16 and 23. The plasma was isolated and IFN-γ levels were measured from duplicate samples using ELISA. 8 animals are included in each group (except Pcb7–13 + 17–23 and tumor-bearing controls day 23; n = 7 and n = 6). Significantly increased IFN-γ levels were detected in animals treated with N32-IFN-γ + Pcb7–13 + 17–23 day 23 compared with day 16 using the Wilcoxon matched pairs test and when compared with animals receiving N32-IFN-γ day 23 using the Mann–Whitney U-test