Fig. 6.

Blocking of DNAM-1 signaling with anti-DNAM-1 or anti-CD155 decreased the effects of TIGIT blockade. (a) Starting on day 10, CIK cells were stimulated with anti-TIGIT or isotype-matched control antibody in the presence of IL-2 for 3 days, and then cultured with at the indicated concentration of anti-DNAM-1 or anti-CD155 antibodies for 2 days. Production of IFN-γ was determined by ELISA. (b) Starting on day 10, CIK cells were stimulated with anti-TIGIT or isotype-matched control antibody in the presence of IL-2 for 3 days, and then cultured with 50 μg/ml anti-DNAM-1 antibodies for 2 days. CIK cells were then incubated with A375, 786-O or K562 cells at an E:T ratio of 10:1, and specific lysis was determined 4 h later. Data are shown as mean ± SD of four replicates. *p < 0.05, **p < 0.001 compared to control group