Fig. 6.

The protective effect of inhibiting RA is associated with the induction of tumour-infiltrating Treg cells. C57BL/6 mice were injected s.c. into the flank with 2 × 10⁵ B16 tumour cells. Mice were injected s.c. into the region of the tumour on days 3, 10 and 17 post-tumour induction with DC vaccine with and without RARi as described in Fig. 5c. The experiment was terminated when tumours reached a diameter of 15 mm. a Representative dot plot of tumour-infiltrating CD4⁺ T cells, CD25⁺FoxP3⁺ T cells (gated on CD4), IL-10⁺ CD4 T cells or IFN-γ⁺ CD4 T cells from each treatment group. b Representative dot plot of tumour-infiltrating IFN-γ⁺ and IL-10⁺ CD8⁺ T cells from each treatment group. c Mean frequency of CD4⁺CD25⁺FoxP3⁺ T cells, CD4⁺IL-10⁺ T cells, CD4⁺IFN-γ⁺ T cells amd CD8⁺IFN-γ⁺ T cells from each treatment group (n = 8 from 2 experiments, *p < 0.05, **p < 0.01 versus untreated; #p < 0.05 versus DC vaccine, by one way ANOVA)