Fig. 1.

Proliferation of stimulated CD4⁺ T cells is abrogated by TPT. CD4⁺ T cells (10⁵/0.2 ml/well) were stimulated in the presence or absence of 1 μM TPT either a polyclonally with anti-CD3 plus anti-CD28 antibodies, or b with allogenic human Mo-DCs (2 × 10⁴/0.2 ml/well) harvested on day 8 of culture, left unstimulated, or stimulated for the last 48 h of culture with a maturation cocktail consisting of IL-1β, TNF-α, and PGE₂. T cell proliferation was measured by incorporation of [³H] thymidine for the last 16 h of culture. a, b Data represent the mean ± SEM of three independent experiments. Statistical significance: a *polyclonally stimulated T cells cultured in the presence of TPT versus w/o TPT, b *T cell stimulated by stimulated versus unstimulated DCs, T cells activated by unstimulated (#) or stimulated (+) allogenic DCs in the presence or absence of TPT (*^,+ P < 0.05, ^## P < 0.01)