Fig. 3.

Augmentation of LPS-induced NF-κB activation by ASAP1 silencing. a and c ASAP1 or control siRNA-transfected cells were treated with LPS (100 ng/ml) for 45 min and the phosphorylation of p65 NF-κB or IKK-β in whole cell extract was analyzed by immunoblotting with anti-phosphorylated form antibody. A typical result of three independent experiments is shown. b ASAP1 or control siRNA-transfected RAW 264.7 cells were further transfected with a luciferase reporter gene and then stimulated with LPS (100 ng/ml) for 6 h and the luciferase activity was determined using a luminometer. *P < 0.01 versus control siRNA