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Effect of ASAP1 silencing on LPS-induced expression of IRAK4, TRAF6, and activation of Akt. ASAP1 or control siRNA-transfected RAW 264.7 cells were stimulated with LPS (100 ng/ml) for 45 min, and the expression of IRAK4 (a), TRAF6 (b), and the phosphorylation of Akt (c) were analyzed with immunoblotting. A typical result of three independent experiments is shown
