Fig. 6.

Augmentation of ASAP1 expression and NO production by various TLR ligands. a RAW 264.7 cells were stimulated with polyI:C (TLR3 ligand, 10 μg/ml), pam3cys (TLR2 ligand, 10 μg/ml), imiquimoid (TLR7 ligand, 10 μg/ml) or CpG-DNA (TLR9 ligand, 2.5 μg/ml) for 30 min. The ASAP1 expression was analyzed with immunoblotting. A typical result of three independent experiments is shown. b ASAP1 or control siRNA-transfected RAW 264.7 cells were stimulated with Pam3cys (10 μg/ml) for 24 h. The level of nitrite in the culture supernatant was determined by Griess reagent. *P < 0.01 versus control siRNA