Fig. 4.

The involvement of TLR1, TLR2 and TLR6 signaling pathways in the production of proinflammatory cytokines in THP-1. a Anti-TLR1, anti-TLR2 and anti-TLR6 antibodies inhibited IL-1β, IL-8 and TNFα production in THP-1 cocultured with SK-OV-3. Pretreatment of THP-1 with or without 10 μg/ml antibody (control IgG1 Ab, anti-hTLR1-IgG, anti-hTLR2-IgG or anti-hTLR6-IgG) for 1 h at 37 °C and then cocultured with SK-OV-3 for 24 h in a transwell culture system. The expression of cytokines in THP-1 was measured by RT-PCR. b The blocking effect of anti-TLR1, anti-TLR2 and anti-TLR6 antibodies on MyD88, TRAF6, TANK, NF-κB p65 and p-NF-κB p65 activation in THP-1 was analyzed by western blot