Table 1.
PCR primers used for the detection of human TLRs and proinflammatory cytokines
| Primers | Sequence (5′ > < 3′) | Length (bp) | MW (bp) | |
|---|---|---|---|---|
| TLR1 | Forward | GGAGGCAATGCTGCTGTT | 20 | 120 |
| Reverse | GCCCAATATGCCTTTGTT ATCCTG | 24 | ||
| TLR2 | Forward | TGTTGCAAGCAGGATCCAAAG | 21 | 157 |
| Reverse | CACAAAGTATGTGGCATTGTCCAG | 24 | ||
| TLR3 | Forward | GGACTTTGAGGCGGGTGTT | 19 | 141 |
| Reverse | TGTTGAACTGCATGATGTACCTTG A | 25 | ||
| TLR4 | Forward | AGGATGATGCCAGCATGATGTC | 22 | 198 |
| Reverse | TCAGGTCCAGGTTCTTGGTTGAG | 23 | ||
| TLR5 | Forward | AAGATGTCGGAGCCTCAGATG | 21 | 181 |
| Reverse | GGGTCCCTGGTTGTTTAAAGACTTC | 25 | ||
| TLR6 | Forward | CAGAGTGAGTGGTGCCATTACGA | 23 | 138 |
| Reverse | AGCCTTCAGCTTGTGGTACTTGTTG | 25 | ||
| TLR7 | Forward | TCTTCAACCAGACCTCTACATTCCA | 25 | 172 |
| Reverse | GGAACATCCAGAGTGACATCACAG | 24 | ||
| TLR8 | Forward | GCGCTGCTGCAAGTTACGGA | 20 | 203 |
| Reverse | TCGACGATTGCTGCACTCTG | 20 | ||
| TLR9 | Forward | GGGACCTCGAGTGTGAAGCA | 20 | 258 |
| Reverse | CTGGAGCTCACAGGGTAGGAA | 21 | ||
| IL-1β | Forward | CACGATGCACCT GTACGATCA | 21 | 120 |
| Reverse | GTTGCTCCATATCCTGTCCCT | 21 | ||
| IL-6 | Forward | AACCTGAACCTTCCAAAGATGG | 22 | 865 |
| Reverse | TCTGGCTTGTTCCTCACTACT | 21 | ||
| IL-8 | Forward | GCCTTCCTGATTTCTGCAGCT | 21 | 868 |
| Reverse | TGCACTGACATCTAAGTTCTTTAGCAC | 27 | ||
| TNFα | Forward | ATGAGCACTGAAAGCATGATCC | 22 | 822 |
| Reverse | GAGGGCTGATTAGAGAGAGGTC | 22 | ||
| β- actin | Forward | TGGCACCCAGCACAATGAA | 19 | 186 |
| Reverse | CTAAGTCATAGTCCGCCTAGAAGCA | 25 | ||