Fig. 1.

Purification and characterization of the Ec-LDP-TF or its enediyne-energized analogue Ec-LDM-TF. a Diagram of NdeI/XhoI gene fragments encoded for the fusion proteins Ec-LDP-TF, LDP-TF, and LDP (top). Bottom, the amino acid sequence of Ec-LDP-TF. b SDS-PAGE (left) and Western blot (right) analysis of fusion proteins. Lane 1 molecular weight marker; Lane 2 LDP protein; Lane 3 LDP-TF protein; Lane 4 Ec-LDP-TF protein. (c) and (d) The purity of LDP-TF and Ec-LDP-TF were analyzed by HPLC on S3000 column, respectively