Fig. 2.

Binding affinity of Ec-LDP-TF protein in vitro and the optical imaging in vivo. a Expression of EGFR on different carcinoma cell lines analyzed by Western blotting. b Binding affinities of three fusion proteins with A431 and J774A.1 cells. Following FACS, the mean fluorescence intensity was plotted versus fusion protein concentration. c Immunorescence assay on binding affinity of Ec-LDP-TF using A431 cells. Red fluorescence located around the cells staining with anti-His-tag antibody (TRITC –conjugated antibody) as well as 4′,6-diamidino-2-phenylindole (DAPI). d Optical imaging in living nude mice bearing A431 xenografts treated with Dylight 680 antibody-labeled Ec-LDP-TF. The images collected at different times after i.v. injection of Dylight 680 antibody-labeled Ec-LDP-TF. Red circles the tumor areas