Fig. 2.

Plasma from CLL patients primes neutrophils from HD to release NETs. There were no differences in ROS production (a), elastase activity (b) or MPO activity (c) between neutrophils from CLL patients and HD donors (mean ± SEM, n = 6 or 10, ns not significant according to Kruskal–Wallis test). d Neutrophils from young HD were incubated with plasma from CLL patients or age-matched HD prior to induction of NETosis. Results are shown as the mean ± SEM, n = 10. Significance was determined using Friedman test followed by Dunn’s multiple comparison posttest. e Representative images of NETotic neutrophils from HD activated in the presence of CLL plasma. Scale bar 15 µm. The percentage of cells showing loss of nuclear lobular shape and co-localization of elastase and DNA (arrow heads) are shown (mean ± SEM of 5 experiments). Significance was determined using Friedman test followed by Dunn’s multiple comparison posttest