Fig. 5.

NETs delay spontaneous apoptosis and increase the expression of activation markers in CLL-B cells. a Shown are representative dot plots and the percentage of Annexin-V-positive cells after 48 h with or without ionomycin-induced NETs. Significance was determined using Wilcoxon matched-pairs test, n = 13. b Expression of activation markers in CLL cells incubated with PMA-induced NETs, supernatants from PFA-fixed neutrophils or medium (mean ± SEM, n = 6–9 CLL). Significance was determined using Friedman test followed by Dunn’s multiple comparison posttest. c Apoptosis and activation markers in CLL cells incubated with medium, supernatants from unstimulated neutrophils or NETs induced by IL-8 + LPS (mean ± SEM, n = 6)