Fig. 1.
Methodical details: influence of “natural” polyreactive antibodies in mouse sera and its elimination. The binding of serum antibodies to carbohydrate antigens was analyzed by ELISA with PAA-glycoconjugates in four mice (M1 to M4) before and after immunization with the TFα non-expressing strain E. coli D-3. a Natural IgM and IgG antibody binding of pre-immune sera to several PAA-glycoconjugates. b IgM and IgG antibody binding of pre- and post–immunization (day 21) sera to TFα and Lec (both absent at the bacterial strain E. coli D-3). c relative level of IgM and IgG TFα specific antibodies (TFαR) of pre- and post-immunization sera. Sera were diluted 1/50–1/100. Single points are either means of duplicates or means of all mice. * p < 0.05 was considered statistically significant. Pre: preimmune serum; post: post-immune serum