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Time course of the development of B16MEL4A5 melanoma tumours in mice, dependent on the number of cells injected. 2 × 104 (filled inverted triangle), 5 × 104 (filled triangle), 1.25 × 105 (filled diamond), 2.5 × 105 (filled square) and 5 × 105 (filled circle) B16MEL4A5 mouse melanoma cells were injected into the right flanks of C57BL/6 J mice. Tumour development and evolution were assessed by measuring their volume with a calliper
