Fig. 3.

GADD34 deficiency exhibited lower cytokine expression and hepatic compensatory proliferation after chronic DEN treatment. a Real-time PCR analysis of mRNA expression levels of the indicated genes in liver from WT and GADD34−/− mice after chronic DEN treatment. Data represent mean ± SEM of three independent experiments, *P < 0.05, ***P < 0.001 versus WT; ns no significance versus WT. b Representative immunofluorescent confocal microscopic images of tissues co-stained for F4/80 and IL-6 in liver tissues after chronic DEN treatment. Nuclei (4′,6-diamidino-2-phenylindole, DAPI), blue; IL-6, green; F4/80, pink. The red outlined areas are enlarged in top left corners. Scale bars 100 μm. c Statistical analysis of mean fluorescence intensity of IL-6 in Kupffer cells/macrophages. N = 3 with >8 fields of view for each group, *P < 0.05 versus WT, mean ± SEM. no significance versus WT. d Representative protein expression levels of liver samples from WT and GADD34−/− mice after chronic DEN treatment. e Analysis of hepatic compensatory proliferation by staining for Ki67. Black arrows indicate Ki67-positive cells. Scale bars 100 μm. Data represent mean ± SEM of three independent samples with >6 fields of view for each sample (LPF, low-power field). ***P < 0.001 versus WT. ns no significance versus WT