Fig. 5.
Treg-mediated suppression of CEA responses in preoperative samples correlate with clinical outcome in patients with metastatic CRC. a–d PBMC and PBMC depleted of regulatory T cells from patients with metastatic CRC were stimulated with different CEA peptides [CEA61–69 H L F G Y S W Y K (a, b) and CEA318-327 T Y A C F V S N L (c, d)] and TNFα (a, c) and IFNγ (b, d) expression determined by flow cytometry in both cell populations. Patients were classified as disease free (n = 8, 18 months) or with recurrent disease (n = 10). Mean ± SEM is shown. Wilcoxon Signed Rank test was used to analyze paired data and Mann–Whitney Rank Sum test to assess differences between groups