Table 2.
Expression of antigen presentation–related molecules on DCs using 3D-culture system
| Necrotic cells(−) N/N-imDCs |
Necrotic cells(−) N/H-imDCs |
Necrotic cells(−) H/H-imDCs |
①Necrotic cells(+) N/N-imDCs |
②Necrotic cells(+) N/H-imDCs |
③Necrotic cells(+) H/H-imDCs |
|
|---|---|---|---|---|---|---|
| CD80 |
29.99 ± 7.36 (13.96 ± 4.35) |
28.75 ± 4.37 (15.12 ± 2.69) |
34.89 ± 10.12 (19.72 ± 4.56) |
65.53 ± 14.16 * (28.02 ± 2.26)** |
37.58 ± 8.29 (12.92 ± 3.41) |
39.17 ± 11.71 (22.71 ± 2.87) |
| CD86 |
31.99 ± 15.1 (45.16 ± 26.36) |
35.25 ± 13.25 (50.3 ± 30.87) |
40.45 ± 10.47 (91.33 ± 33.73) |
75.66 ± 6.87 (235.83 ± 79.61)** |
70.51 ± 3.21 (155.17 ± 57.71) |
70.92 ± 5.95 (163.42 ± 31.19) |
| HLA-ABC |
97.97 ± 1.24 (147.59 ± 60.77) |
98.26 ± 0.78 (108.01 ± 35.61) |
97.65 ± 1.26 (167.46 ± 53.22) |
97.69 ± 2.18 (291.10 ± 87.83)** |
98.18 ± 0.84 (127.62 ± 41.69) |
97.84 ± 1.62 (185.27 ± 66.52) |
| HLA-DR |
57.22 ± 5.81 (44.96 ± 7.76) |
62.77 ± 3.29 (45.32 ± 12.32) |
85.17 ± 13.79 (100.49 ± 21.86) |
90.24 ± 6.18 (135.28 ± 54.23)** |
92.42 ± 4.42 (112.83 ± 31.12) |
92.05 ± 5.47 (118.90 ± 43.59) |
| CD83 |
4.52 ± 0.86 (6.61 ± 2.27) |
3.03 ± 1.3 (5.72 ± 1.85) |
7.05 ± 3.48 (6.71 ± 2.78) |
27.44 ± 5.86* (19.59 ± 3.29)** |
4.24 ± 1.72 (10.69 ± 5.57) |
7.42 ± 5.92 (12.98 ± 3.06) |
Expression of CD80, CD86, CD83, HLA-ABC, and HLA-DR was analyzed by FACS analysis. DCs were harvested from the collagen matrix and analyzed on day 5
Data are presented as the percentage of positive cells of three independent donors (MFI of each marker)
* P < 0.05 (① versus ②, ③: percentage of positive cells)
** P < 0.05 (① versus ②, ③: MFI)