Fig. 2.

Treg increases in spleen of B16-F10 tumor-bearing mice. Female C57BL/6 J mice received subcutaneous injection of B16.F10 melanoma cells (2 × 10⁴ cells/mouse), and tumor growth was monitored. a Flow cytometric analysis was performed to identify CD4⁺CD25⁺Foxp3⁺ Treg in spleen of tumor-bearing mice (n = 10) and tumor-free mice (n = 10). b Treg frequency in spleen positively correlates with tumor weight in tumor-bearing mice (n = 10). The correlation between Treg frequency and tumor weight was analyzed using Pearson correlation analysis. **P < 0.01. Data show mean ± SD from a representative experiment that was repeated two times with similar results