Fig. 6.

Poly(I:C) indirectly induces chemotaxis of Th1 cells. a Polarization of human CD4⁺ T cells toward Th1 cells was verified by CXCR-3 surface expression using flow cytometry. One representative example is depicted. b Th1 cells were allowed to migrate for 3 h at 37 °C toward supernatants of monocytes co-cultured with FaDu cells. FaDu cells had been treated with poly(I:C) or imiquimod, if indicated. The mean ± SD of three independent experiments are shown. P value (t test) is indicated. c Th1 cells were allowed to migrate for 3 h at 37 °C toward supernatants of monocytes co-cultured with poly(I:C)-treated FaDu cells. Neutralizing anti-CXCL-10 antibody or isotype control antibody was added to the supernatants if indicated. The mean ± SD of three independent experiments are shown. P value (t test) is indicated