Fig. 3.

Combined cryoimmunotherapy induces anti-metastatic immunity. a Representative images of in vivo D122-luc-5.5 tumor metastasis monitored 57 and 72 days following tumor inoculation (captured with an IVIS 200 imaging system). Luciferase activity 57 (b) and 72 (c) days following tumor inoculation. d Lungs weight, as an indication for metastatic load, was detected 100–120 days post-amputation or when mice were moribund. The data are presented as mean lungs weight (mg). Lungs weight of naïve 25-week-old mouse is 200 mg. Combined results of five independent experiments (n = 5–8 mice per group per experiment). *P < 0.05; **P < 0.001; ***P < 0.0001