Fig. 3.

Subpopulations of myeloid-derived suppressor cells are increased in gastric cancer patients (staining Panel-3). PBMCs from 25 gastric cancer patients (filled circles) and eight healthy donor samples (filled triangles) were analyzed with regard to frequency of various MDSC subpopulations determined as: MDSC1 CD14⁺CD124⁺ (a), MDSC2 CD15⁺CD124⁺ (b), MDSC3 CD33⁺HLA-DR⁻SSC^int (c), MDSC4 CD14⁺HLA-DR^lo (d), MDSC5 CD11b⁺CD15⁺CD14⁻ (e) and MDSC6 CD15⁺FSC^loSSC^hi cells (f). The horizontal black lines represent median. p values were obtained with a Mann–Whitney test, **p < 0.01; ***p < 0.001. HD healthy donors, MDSCs myeloid-derived suppressor cells, n.s. not significant, P patients, PBMCs peripheral blood mononuclear cells