Fig. 1.

Murine Foxp3-expressing CD8 T cells are distinct from their Foxp3− counterparts and unevenly distributed in homeostatic animals. a Representative flow cytometry analysis of CD8 Foxp3⁺ T lymphocyte distribution in lymphocyte populations isolated from spleen, peripheral blood mononuclear cells (PBMCs), and axillary-brachial lymph nodes (AB.LNs) (left panels). Cumulative analysis is expressed as the percentage of total lymphocytes (right panel). *P < 0.05 versus splenocytes. b Representative flow cytometry analysis of CD8 Foxp3+ T lymphocytes (solid histogram) “activated phenotype” using antibodies against CD4 and activation markers (CD25, CD28, CD44). c Representative flow cytometry analysis of CD8+ Foxp3+ T cells (solid histogram) and Foxp3⁻ counterpart (dashed histogram), “immunosuppressive phenotype” in splenic lymphocytes using antibodies against PD-1, NKG2A, and CTLA-4