Fig. 6.

CD8 Foxp3+ T lymphocytes constitutively express immunosuppressive markers, skin-homing chemokine receptors, and integrin. a Representative flow cytometry analysis demonstrating CD8 + Foxp3+ T lymphocytes constitutively express immunosuppressive markers. Lymphocytes were isolated from spleen, peripheral blood mononuclear cells (PBMCs), and axillary-brachial lymph nodes (AB.LNs) from B16-F10 tumor-bearing C57BL/6 mice treated with high-dose rhIL-2 (50,000 U) s.q. or i.p. Cells were stained for CD8, Foxp3 and PD-1, NKG2A, CTLA-4 expression. b Representative flow cytometry analysis demonstrating CD8 + Foxp3+ T lymphocyte constitutive expression of skin-homing chemokine receptors and integrin. Lymphocytes were isolated from spleen, PBMCs, and AB.LNs from B16-F10 tumor-bearing C57BL/6 mice treated with high-dose rhIL-2 (50,000 U) s.q. or i.p. Cells were stained for CCR4, CCR10 CXCR3, or CD103 expression