Fig. 4.
Effects of pretreatment of CTL clones with DAC on antigen-specific killing. a–e Chromium release cytotoxicity assays in which target cells and/or PRA100−108-specific CTL Line 7 were pretreated with DAC for 72 h. Cell lines were pulsed with peptides (HA1, PAX5 or PRA100−108) HA1 and PAX5 are non-specific HLA-A0201 binding peptides, class I denotes pretreatment of target cells with a pan-class-I blocking antibody, whereas Ig denotes its isotype control. All chromium assays performed over 4 h apart from a) (18 h)