Table 1.
Expression of PRAME mRNA in human cancer cell lines and normal tissues, and HLA-A0201 protein expression by flow cytometry in cell lines used in this study
| Tissues and tumour cell line | % of PRAME mRNA expression (Standard deviation) relative to RH18 | Type of cell line | HLA-A0201 expression |
|---|---|---|---|
| Cancer cell lines | |||
| BV173 | 1.5 (0.6) | Pre-B leukaemia | ++ |
| BJAB | 99 (3) | Pre-B leukaemia | ++ |
| Jekka | 20 (0.8) | Pre-B leukaemia | ++ |
| NALM-1 | 0.1 (0.001) | Pre-B leukaemia | + |
| NALM-6 | 0.2 (0.001) | Pre-B leukaemia | + |
| NALM-17 | 2 (0.2) | Pre-B leukaemia | + |
| NALM-20 | 3 (0.3) | Pre-B leukaemia | + |
| NALM-27 | 1.2 (0.3) | Pre-B leukaemia | + |
| REH | 32 (0.2) | Pre-B leukaemia | − |
| K562 | 312000 (20) | Chronic myelogenous leukaemia | − |
| Mott 4 | 132 (3.6) | Acute T-cell leukaemia, | − |
| HL60 | 110 (6) | Acute promyelocytic leukaemia | − |
| Daudi | 14.9 (1) | Burkitt’s lymphoma | − |
| Raji | 0.3 (0.1) | Burkitt lymphoma | − |
| U937 | 70.8 (0.2) | Histiocytic lymphoma | − |
| MDA-MB-231 | 1.9 (0.1) | Breast adenocarcinoma | +++ |
| ZR-1-75-1 | 1.4 (0.1) | Breast adenocarcinoma | − |
| MCF-7 | 145 (5.6) | Breast adenocarcinoma | ++ |
| SHSY5Y | 241 (5) | Neuroblastoma | − |
| LAN-1 | 110 (6) | Neuroblastoma | − |
| SK-N-AS | 0.1 (0.1) | Neuroblastoma | − |
| TC32 | 21 (2.1) | Ewing sarcoma | ++ |
| RH1 | 26 (1.5) | Ewing sarcoma | + |
| RH18 | 100 (1) | Rhabdomyosarcoma | ++ |
| SW480 | 1.2 (0.2) | Colon adenocarcinoma | ++ |
| SW1116 | 8.9 (0.5) | Colon adenocarcinoma | − |
| Colo205 | 1.3 (0.1) | Colon adenocarcinoma | + |
| SK-col-1 | 107 (3) | Colon adenocarcinoma | − |
| SW403 | 184 (7.9) | Colon adenocarcinoma | ++ |
| Non-malignant cell line | |||
| T2 | 0.2 (0.01) | TAP-deficient Lymphoblastoid cell | ++ |
| Primary cells | |||
| G-7 | 2 (0.4) | Acute lymphoblastic leukaemia | ++ |
| Normal tissue | |||
| Testis | 17.20 (0.01) | ||
| Kidney | 13.4 (0.031) | ||
| Bone marrow | 5.95 (0.025) | ||
| Liver | 2.35 (0.001) | ||
| Salivary gland | 2.35 (0.001) | ||
| Prostate | 2.3 (0.013) | ||
| Spleen | 2.3 (0.1) | ||
| Brain | 1.2 (0.01) | ||
| Skeletal muscle | 1.2 (0.01) | ||
| Small intestinal | 1.18 (0.012) | ||
| Stomach | 0.7 (0.021) | ||
| Colon | 0.66 (0.001) | ||
| Lung | 0.6 (0.022) | ||
| Heart | 0.1 (0.011) | ||
| Placenta | 0 (0.001) | ||
Percentage PRAME expression is relative to RH18