Fig. 1.

LPS-stimulated PBMC media increased the production of IL-10 by melanoma cells. a IL-10 levels after co-culture with PBMC-derived media. CM005 melanoma cells were cultured with culture supernatants from unfractionated PBMC without LPS (control) or non-adherent, adherent, and unfractionated PBMC with 10 ng/ml of LPS for 2 days, and then, the supernatants were collected. The IL-10 productions in supernatants were analyzed by the ELISA assay. The average of duplicates in a representative experiment was shown. b Cytokine levels in conditional culture media. The average of duplicates in a representative experiment was shown