Fig. 6.

Inhibitions of IL-6 pathways to block IL-10 production by CM005 melanoma cells. a Inhibition of IL-6-induced IL-10 productions by various kinase inhibitors. CM005 cells were treated with various kinase inhibitors 30 min prior to rhIL-6 (2 ng/ml) stimulation. After 24 h of incubation, the production of IL-10 in the supernatant was analyzed by ELISA assay. The data were shown as a fraction in comparison with IL-10 production in control (Ctl, no inhibitor). *P < 0.05. b Suppression of STAT3 and ERK1/2 phosphorylation by kinase inhibitors. After treatment with individual kinase inhibitors followed by IL-6 stimulation, CM005 cells were lysed and phosphorylation of STAT3 (left) and ERK1/2 (right) was measured by Western blot. The intensity of bands for phosphorylated STAT and ERK1/2 was compared to that of control (Ctl, no inhibitor). *P < 0.05, **P < 0.01