Fig. 3.
HLA class II restrictions and optimal epitopes recognized by the two clones. a Clones 1A3 and 1C9 were stimulated by MELOE-132–46 and MELOE-126–40, respectively, in the presence or not of decreasing concentrations of anti-HLA-DP, -DQ or -DR mAbs. b The HLA class II-restricting alleles were determined by assessing 1A3 and 1C9 TNF- α responses against unpulsed (grey histogram) or peptide-pulsed (dotted line) EBV-B cells expressing HLA-DQ or -DR alleles from the donors DA3 and DC1. c Clone 1C9 was incubated at a 1:1 ratio with the HLA-DRβ1*1101+ C3 EBV-B cell-line loaded with various concentrations of the indicated peptides and TNF production was measured in the WEHI biological assay. d As in c with clone 1A3 and the HLA-DQβ1*0603+ BL30/95 EBV-B cell-line