Fig. 4.
Functional characterization of the CD4+ T cell clones. a Cytotoxicity of CD4+ T cell clones 1A3 and 1C9 against peptide-pulsed EBV-B cells was assessed by a standard chromium release assay. b Representative examples of HLA-DQ or HLA-DR expression by melanoma cell-lines after incubation with (bold line) or without (thick line) 500 IU/ml IFN-γ for 48 h. c IFN-γ production by CD4+ T cell clones 1A3 and 1C9 in response to HLA class II-matched melanoma cell-lines, treated or not with IFN-γ and loaded or not with MELOE-1 peptides. Shown are representative responses of 1A3 and 1C9 T cell clones toward one out of three HLA-DQβ1*0603+ and one out of five HLA-DRβ1*1101+ MELOE-1-expressing melanoma cell-lines tested, respectively. d IFN-γ production by clone 1A3 in response to HLA-matched DC loaded with M117 or SW480 cell lysate or the MELOE-132–46 peptide as positive control (n = 2)