Fig. 1.
Expression of SAP gene in the tumor and liver of mice treated with PDT vaccine. Mice bearing SCCVII tumors were treated with either PDT vaccine or control vaccine using SCCVII cells exposed only to X-rays (60 Gy). The vaccine was prepared by incubating SCCVII cells with ce6 (0.5 μg/ml) for 30 min followed by exposure to 665 ± 10 nm light (1 J/cm2) and then 16-h post-incubation at 37°C. The cells were then collected, exposed to X-rays (60 Gy), and injected into SCCVII tumor-bearing mice (2 × 107 cells per mouse peritumorally). The mice were killed at 24 h after vaccination and their tumor and liver tissues collected for quantitative RT-PCR-based analysis of the expression of SAP gene. The results are presented as GAPDH-normalized SAP gene expression relative to that in the same tissue of untreated tumor-bearing mice. Each treatment group consisted of 4 mice; bars represent standard deviations. *Statistically significant difference in response (P < 0.05) compared to the unvaccinated control group; **statistically significant difference in response (P < 0.05) compared to control vaccine group