Fig. 5.

Progressive disease is associated with an upregulation of PD-1 and with dysfunctional T cells. Percentages of PD1⁺ CD4⁺ T cells and PD1⁺ CD8⁺ T cells (a), LAG3⁺ CD4⁺ and LAG3⁺ CD8⁺ T cells (b) and TIM3⁺ CD4⁺ and TIM3⁺ CD8⁺ T cells (c) determined in the absence or presence of anti-CD3/CD28 beads at baseline and at 6 weeks for patients with SD/PR (filled circle; n = 7) or PD (inverted triangle; n = 8). IFN-γ, TNF-α, IL-2 and IL-10 were quantified in the supernatants in the absence or presence of anti-CD3/CD28 beads at baseline and at 6 weeks for patients with SD/PR (filled circle; n = 7) or PD (inverted triangle; n = 8) (d)