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Cytotoxicity against HLA-class I-matched cancer cells. Pre- and post-vaccination peripheral blood mononuclear cells (PBMCs) were cultured with interleukin-2 and the corresponding peptide for 14 days. The cytotoxicity of cultured PBMCs against Colo201 (HLA-A2+ colon carcinoma), SW620 (HLA-A2- and -A24+ colon carcinoma), Colo320 (HLA-A24+ colon carcinoma), and phytohemagglutinin (PHA)-blastoid T cells (HLA-A2+ or HLA-A24+) was measured
